Understanding the Western Blot
نویسنده
چکیده
Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. Among the most commonly asked questions are: What laboratory techniques are used to carry out the assay? What exactly is being measured? What is a “band”? How are the results interpreted? What are the CDC criteria for a “positive” test? Although some of the medical jargon associated with immunology can be a little overwhelming, the scientific principles behind these tests are not difficult to grasp. The following article is offered as a primer in the techniques and interpretation of Western blotting, and should help most patients navigate their way through some of the medical and scientific terminology associated with the assay.
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